Characteristic of K3 (CpG-ODN) as a Transcutaneous Vaccine Formulation Adjuvant

Characteristic of K3 (CpG-ODN) as a Transcutaneous Vaccine Formulation Adjuvant

Blog Article

Transcutaneous immunization (TCI) is easy to use, minimally invasive, and has excellent efficacy in vaccines against infections.We focused on toll-like receptor (TLR) ligands as applicable adjuvants for transcutaneous formulations and characterized immune responses.TCI was performed using poke-and-patch methods, in which puncture holes are formed with a here polyglycolic acid microneedle on the back skin of mice.

Various TLR ligands were applied to the puncture holes and covered with an ovalbumin-loaded hydrophilic gel patch.During the screening process, K3 (CpG-oligonucleotide) successfully produced more antigen-specific antibodies than other TLR ligands and induced T helper (Th) 1-type polarization.Transcutaneously administered K3 was detected in draining lymph nodes and was found to promote B cell activation and differentiation, suggesting a direct transcutaneous adjuvant activity on B cells.

Furthermore, a human safety test of K3-loaded self-dissolving microneedles (sdMN) was performed.Although a local skin reaction was observed at the sdMN application site, there was no systemic side reaction.In summary, we report a K3-induced Th1-type immune response that is caruso rhodiola a promising adjuvant for transcutaneous vaccine formulations using MN and show that K3-loaded sdMN can be safely applied to human skin.